rabbit polyclonal primary antibodies against insulin receptor β subunit  (Cell Signaling Technology Inc)

Journal: Metabolism: clinical and experimental

Article Title: Hepatic insulin-degrading enzyme regulates glucose and insulin homeostasis in diet-induced obese mice

doi: 10.1016/j.metabol.2020.154352

Figure Lengend Snippet: Effect of hepatic IDE production on insulin signaling pathways and the insulin-IR-complex. (A) Representative western blots depicting the effects of insulin on total or phosphorylated IR, AKT1, AKT2, GSK3α, and GS. Expression of GAPDH was determined to ensure similar protein levels for total liver lysates. Two representative samples have been used for the final arrangement of the figure. Densitometric analyses of the data in panel A. The y-axis represents the ratio of total protein or phosphorylated versus GAPDH, and phosphorylated versus total protein in arbitrary units (AU) for IR (B), p-IR (C), p-IR/IR (D), AKT1 (E), p-AKT1Ser473 (F), p-AKT1Thr308 (G), p-AKT1Ser4731/AKT1 (H), p-AKT1Thr308/AKT1 (I), AKT2 (J), p-AKT2Ser474 (K), p-AKT2Ser474/AKT2 (L), GSK3α (M), p-GSK3α (N), p-GSK3α/GSK3α (O), GS (P), p-GS (Q), p-GS/GS (R) in WT and L-IDE-KO mice. Data are mean ± SEM. n = 3–6 per genotype and condition. * p value <0.05, $ p value <0.05 by ANOVA. Co-immunoprecipitation of IDE and IR. Aliquots of liver lysates from mice transduced with Ad.null or Ad.IDE vectors were subjected to immunoprecipitation with α-IR followed by immunoblotting with α-IDE antibody. (S) Western blot images. (T) Densitometric quantification of co-immunoprecipitation of IDE and IR. Data are mean ± SEM n = 3 independent experiments. * p value <0.05 by Student’s t -test.

Article Snippet: For co-immunoprecipitation experiments, polyclonal antibodies against IDE (#AB9210, Millipore, USA) and insulin receptor (#3025, Cell Signaling, USA) were used to immunoprecipitate proteins from tissue lysates as previously described [ ].

Techniques: Protein-Protein interactions, Western Blot, Expressing, Immunoprecipitation, Transduction